Informatics Publishing Limited

E. B. Onuigbo ¹, V. C. Okore ¹, A. A. Ngene ², C. O. Esimone ³, A. A. Attama ¹

Affiliations
1 Department of Pharmaceutics, University of Nigeria, Nsukka, 410001, Nsukka, NG
2 Department of Veterinary Medicine, University of Nigeria, Nsukka, NG
3 Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, NG

Abstract

Cationic liposomes are the leading drug delivery systems and are gaining increasing importance in gene therapy as an alternative to recombinant viruses. This article is a preliminary work on the characterization and in vitro study of a stearylamine-based cationic liposome. Multilamellar vesicles were prepared using the lipid film hydration technique. Vesicle morphology was evaluated by digital light microscopy. Particle size, zeta potential and polydispersity were determined using photon correlation spectroscopy. Susceptibility of Staphylococcus aureus to amoxicillin encapsulated in stearylamine-based cationic liposome was compared to 1,2-dioleoyl-3-trimethylammonium propane (DOTAP)-based cationic liposome and the marketed drug. The vesicles of the stearylamine based cationic liposome were spherical and concentric with a core. Particle size distribution had a peak at 94 nm, zeta potential was 34 mV and polydispersity was greater than 0.3. The clinical isolate of Staphylococcus aureus was most susceptible to stearylaminecationic liposome with an inhibitory zone diameter (IZD) of 25.00 mm+0.58>DOTAP-based cationic liposome with IZD of 24.67mm+0.67>tablet amoxicillin with IZD of 23.00 mm+0.58.

Keywords

Stearylamine, Cationic Liposome, Vesicles, Amoxicillin, Staphylococcus Aureus.

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